|
This research was carried out to investigate antimutagent effect of peach enzymatic browning reaction products(PEBRP) obtained by reacting each of polyphenol compounds with oxidase extracted from Korea-cultivated peach. In methods, rec-assay with B. subtilis strains H17(rec^+ ) and M45(rec^+ ), and Ames test with S. typhimurium TA98 and TA100 were used. The spore rec-assay of PEBRP, pyrogallol, hydroxyhydroquinone, homocatechol and caffeic acid were not showed mutagenicity. In the effects of various metal ions(A1^(3+), Cu^(2+), Fe^(2+), Mn^(2+), Ni^(2+), Pb^(2+), Zn^(2+)) on the rec-assay, all PEBRP except caffeic acid was increased inhibition zone(5 ㎜) only with Zn^(2+). In paticular, the Py-PEBRP was decreased the difference of inhibition zone of growth on MMC(mitomycin C). In results of Ames test, all PEBRP were not showed mutagenicity on S. typhimurium TA98 and TA100; however, Ca-PEBRP and Hca-PEBRP were suppressed mutagenic effects on Trp-P-1 and B(a)P in the presence of S-9Mix.
|